EDUCATION AND EMPLOYMENT:

B.Sc. in Zoology: Visva-Bharati, Santiniketan, WB (1997-2000)

M.Sc. in Zoology: Visva-Bharati, Santiniketan, WB  (2000-2002)

Ph.D. in life Science: Visva-Bharati, Santiniketan, WB (2003-2008)

Research Associate: USUHS, Bethesda, MD, USA (2008-2012)

Scientist: CSIR-IITR, Lucknow, UP (2012-cont.)

RESEARCH FOCUS OF THE LAB:

Microglial cells are modified macrophages that protect central nervous system (CNS) from infections and other assaults to it. Its activities begin in the early weeks after birth and continue thereafter. Effect on its development and functions in early life can therefore be detrimental to neuronal activity of affected individual. Microglia acts as double edged sword. In resting state microglia helps in neuronal survival by secreting trophic factors (BDNF, IGF) and anti-inflammtory molecules (IL-10 and TGF-β). On the other hand, over activation of microglia exerts detrimental effect by releasing high amount of pro-inflammatory factors like superoxide, nitric oxide, IL-1β, IL-6 as well as TNF-α.  

Environmental chemicals, such as lead, trimethyltin and diesel exhaust potentially hyperactivate microglia and induce neuronal anomalies. Our lab is focused to find out the molecular mechanisms of xenobiotic induced altered immune function of microglia.

PUBLICATIONS:

#  Peer reviewed articles:

1.  Jamal Ahmad Ansari, Shubhendra K. Mishra, Rajib K. Dey, Opalina Roy, Shaivya Kushwaha, Vikas Singh, Satyakam Patnaik, Ghosh D*. (2022) Minocycline reverses developmental arsenic exposure-induced microglia activation and functional alteration in BALB/c mice. Environ. Toxicol. Pharmacol (ETAP).92, 103858 IF: 4.860 [* corresponding author] 

2.     Vikas Singh, Shaivya Kushwaha, Jamal A. Ansari, Siddhartha Gangopadhyay, Ashok K. Giri, Satyakam Patnaik, Ghosh D*. (2022) MicroRNA-129-5p-regulated microglial CD200R1 expression controls neuroinflammation (2022). J Biol Chem. 298(1):101521. IF: 5.157 [* corresponding author]

3.  Choubey A, Girdhar K, Kar AK, Kushwaha S, Yadav MK, Ghosh D, Mondal P (2020) Low-dose naltrexone rescues inflammation and insulin resistance associated with hyperinsulinemia. J Biol Chem. doi: 10.1074/jbc.RA120.013484. IF: 4.24

4.    Kar AK, Singh A, Dhiman N, Purohit MP, Jagdale P, Kamthan M, Singh D, Kumar M, Ghosh D, Patnaik S (2019) Polymerassisted in situ synthesis of silver nanoparticles with epigallocatechin gallate (EGCG) impregnated wound patch potentiate controlled inflammatory responses for brisk wound healing. International Journal of Nanomedicine. 14, 9837-9854. Impact Factor: 4.47 

5.    Singh A, Dhiman N, Kar AK, Singh, D, Purohit MP, Ghosh D, Patnaik S (2019) Advances in Controlled Release Pesticide Formulations: Prospects to Safer Integrated Pest Management and Sustainable Agriculture. J Haz Mat. 385, 121525. Impact Factor: 7.65 

6.    Daniel P. V, Kamthan M, Gera R, Dogra S, Gautam K, Ghosh D and Mondal P.(2019) Chronic exposure to Pb2+ perturbs ChREBP transactivation and coerces hepatic dyslipidemia. FEBS Lett. 593(21):3084-309. doi: 10.1002/1873-3468.13538. Impact Factor: 2.67  

7.   Dogra S, Kar AK, Girdhar K, Daniel VP, Chatterjee S, Choubey A, Ghosh S, Patnaik S, Ghosh D*, Mondal P* (2019). Zinc oxide nanoparticles attenuate hepatic steatosis development in high-fat-diet fed mice through activated AMPK signalling axis. Nanomedicine: Nanotechnology, Biology, and Medicine. 17, 210-222. doi: 10.1016/j.nano.2019.01.013. Impact Factor: 6.5  [* corresponding author]

8.    Singh V, Kushwaha S, Gera R, Ansari A J, Mishra J, Dewangan J, Patnaik S, Ghosh D*. (2017) Sneaky Entry of IFNγ Through Arsenic-Induced Leaky Blood–Brain Barrier Reduces CD200 Expression by Microglial pro-Inflammatory Cytokine. Mol Neurobiol. https://doi.org/10.1007/s12035-018-1155-0.  Impact Factor: 6.19  [* corresponding author]

9.  Purohit MP, Verma NK, Kar AK, Singh A, Ghosh D, Patnaik S. (2017) Inhibition of Thioredoxin Reductase by Targeted Selenopolymeric Nanocarriers Synergizes the Therapeutic Efficacy of Doxorubicin in MCF7 Human Breast Cancer Cells. ACS Appl Mat Inter. 9; 36493-36512. Impact Factor: 7.504    

10.  Dey G, Singh V, Dewangan J, Daniel P V, Kamthan M, Ghosh D*, Mondal P*, Ghosh S*. (2017) Renal clearable new NIR probe: precise quantification of albumin in biofluids and fatty liver disease state identification through tissue specific high contrast imaging in vivo. Anal Chem. 89; 10343-10352. Impact Factor: 6.32  [* corresponding author]

11.  Gera R, Singh V, Mitra S, Sharma AK, Singh A, Dasgupta A, Singh D, Kumar M, Jagdale P, Patnaik P, Ghosh D*. (2017) Arsenic exposure impels CD4 commitment in thymus and suppresses T cell cytokine secretion by increasing regulatory T cells. Sci. Rep. 7; 7140. Impact Factor: 4.259  [* corresponding author]

12.   Singh V, Gera R, Purohit MP, Patnaik S, Ghosh D*. (2017)  Fluorometric estimation of Glutathione in Cultured Microglial Cell Lysate. Bio-protocol. 7, 1-9, DOI:10.21769/BioProtoc.2304. IF: NA [* Corresponding author]

13. Sharma AK; Singh V; Gera R; Purohit MP; Ghosh D*. (2016) Zinc Oxide Nanoparticle Induces Microglial Death by NADPH-Oxidase-Independent Reactive Oxygen Species as well as Energy Depletion. Mol Neurobiol.  DOI 10.1007/s12035-016-0133-7. Impact Factor: 5.397 [* corresponding author]

14. Singh V, Gera R, Kushwaha R, Sharma AK, Patnaik S, Ghosh D*. (2016) Hijacking microglial glutathione by inorganic arsenic impels bystander death of immature neurons through extracellular cystine/glutamate imbalance. Sci. Rep. 6; 2016; 30601. Impact Factor: 5.228 [* corresponding author]

15.  Singh V, Mitra S, Sharma AK, Gera R, Ghosh D*. (2014) Isolation and characterization of microglia from adult mouse brain: Selected applications for ex-vivo evaluation of immunotoxicological alterations following in-vivo xenobiotic exposure.. Chem Res Toxicol. 27, 895−903. IF: 4.1 [* corresponding author]

*Impact factors given as on date of publication

# Book Chapter:

1.  Gera R, Singh V, Sharma AK, Ghosh D*. (2015) Environmental Chemicals: Insights into Mammalian Immune Responses to Arsenic, Cadmium, and Lead.  Adv Mol Tox. vol.9, 109-159. [Book series published by ELSEVIER]. 

2.   Kushwaha S, Sharma AK, Singh V, Gera R, Ansari J, Ghosh D*. (2018) माइक्रोग्लिया के स्वास्थ्य एवं कार्यशैली पर नैनोकणों का प्रभाव (Effect of nano-particle on microglial health). Hindi Book “Vishvigyan Ke Naye Aayaam (New dimension of toxicology research)”. 1^st Edt. Editors: Dr. D. Kar Chowdhuri and Prof. Alok Dhawan, CSIR-Indian Institute of Toxicology Research. Book pblished by CSIR-Indian Institute of Toxicology research, Lucknow, India. [* corresponding author]. 

[* denotes corresponding author]

Google scholar citation: https://scholar.google.co.in/citations?user=M93FvJIAAAAJ&hl=en

PRESENT LAB MEMBERS AND RESEARCH INTEREST:

Mr. Jamal Ahmad Ansari, UGC- Senior Research Fellow.

Area of research: Arsenic-induced Microglial priming and neuroinflammation.

Ms. Shaivya Kushwaha, CSIR- Senior Research Fellow

Area of research: Arsenic-induced altered signaling and neuroinflammation.

Mr. Shubhendra Kumar Mishra, Project Fellow

Area of research: Nano-carrier mediated drug delivery.

Rajib Kumar Dey, CSIR-Senior Research fellow

Area of research: Stress-induced micro-RNAs (miRNAs) and microglial function.

FORMER LAB MEMBERS:

Mr. Aditya K Kar (Assistant Professor at Adamas University, Kolkata, India)

GROUP PHOTO OF LAB MEMBERS:

From left: Mahaveer, Shaivya, Ruchi, D. Ghosh, Jamal, Aditya, Vikas (June 2018)

RESEARCH COLLABORATOR:

Dr. Satyakam Patnaik, Scientist, CSIR-IITR, Lucknow, UP, India.

Dr. Prosenjit Mondal, Assistant Professor, IIT-Mandi, Mandi, HP, India.

Dr. Arunava Dasgupta, Scientist, CSIR-CDRI, Lucknow, UP, India.

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